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Cell Technology Suite
The Cell Technology Suite provides a dedicated environment for the isolation and analysis of single cells. The Suite is situated within the School of Biological and Biomedical Sciences and comprises three laboratories: • Cell Isolation • Cell Imaging • Gene Expression Within the Cell Isolation Lab we have two Laser Capture microscopes: a PALM MicroBeam and an ARCTURUS PIXCELL II system. These state-of-the-art instruments make it possible to dissect and collect individual cells from tissue sections. Following capture, RNA can be isolated for downstream transcriptomic analysis. DNA isolation from archived sections is also possible. To allow sample preparation on site a Leica cryostat and a tissue processor and embedding station are available. The Cell Imaging Lab is equipped with a controlled environment Zeiss Axiovert withAxioCam and Axiovision software, allowing the movement and development of live cells to be visualised over time. Tissue culture facilities are available on site for cell culture preparation. Live cells can also be characterized by Fluorescence Activated Cell Sorting on our FACS Calibur (BD Biosciences). Micromanipulation of live cells and tissues are possible with an Eppendorf TransferMan. The Gene Expression Lab is equipped with two Realtime PCR machines; a 96 wellplate system (Applied Biosystems) and a RotorGene system (Corbett). The suite is well equipped with peripheral equipment to allow samples and specimens to be prepared on site.
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